Dual-color dual-hapten in situ hybridization (D-DISH) – Comparison with fluorescence in situ hybridization (FISH) for HER2/neu testing in breast cancer

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Comparison of fluorescence in situ hybridization (FISH) and dual-ISH (DISH) in the determination of HER2 status in breast cancer.

The determination of HER2 amplification is critical to selecting appropriate patients for HER2 targeted therapy in breast cancer. Dual in situ hybridization (DISH), an alternative to fluorescence in situ hybridization (FISH) and immunohistochemistry, is now available. To compare the FISH and DISH methods, we tested 251 samples enriched for common or difficult-to-assess HER2 anomalies. Seven sam...

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BACKGROUND Chromogenic in situ hybridization (CISH) is fast becoming a well established technique for easy and sensitive determination of HER2 gene status in breast cancer. However, for the chromogenic method to achieve status as a safe and reliable technique, the method needs to be validated against already known and validated FISH techniques. METHODS Here it is reported from a comparative s...

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Amplification of the HER2/neu gene in 18-20% of patients with breast cancer produces the primary mechanism of HER2 protein overexpression (PenaultLlorca et al., 2009; Thang et al., 2011; Dekker et al., 2012). The HER2 gene is a tumor marker associated with rapid tumor growth, increased recurrence rate after surgery, and decreased life expectancy (Penault-Llorca et al., 2009; Thang et al., 2011)...

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ژورنال

عنوان ژورنال: Indian Journal of Pathology and Microbiology

سال: 2020

ISSN: 0377-4929

DOI: 10.4103/ijpm.ijpm_861_19